Rapid Detection of Subtype H10N8 Influenza Virus by One-Step Reverse Transcription-Loop-Mediated Isothermal Amplification Methods.

Bao, Hongmei; Feng, Xiaoxiao; Ma, Yong; Shi, Jianzhong; Zhao, Yuhui; Gu, Linlin; Wang, Xiurong; Chen, Hualan

Bao, Hongmei; Feng, Xiaoxiao; Ma, Yong; Shi, Jianzhong; Zhao, Yuhui; Gu, Linlin; Wang, Xiurong; Chen, Hualan.

Afiliação

Bao H; Animal Influenza Laboratory of the Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China.
Feng X; Animal Influenza Laboratory of the Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China.
Ma Y; Animal Influenza Laboratory of the Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China.
Shi J; Animal Influenza Laboratory of the Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China.
Zhao Y; Animal Influenza Laboratory of the Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China.
Gu L; Animal Influenza Laboratory of the Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China.
Wang X; Animal Influenza Laboratory of the Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China wxr@hvri.ac.cn chenhualan@caas.cn.
Chen H; Animal Influenza Laboratory of the Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China wxr@hvri.ac.cn chenhualan@caas.cn.

J Clin Microbiol ; 53(12): 3884-7, 2015 Dec.

Article em En | MEDLINE | ID: mdl-26378283

ABSTRACT

ABSTRACT

We developed hemagglutinin- and neuraminidase-specific one-step reverse transcription-loop-mediated isothermal amplification assays for detecting the H10N8 virus. The detection limit of the assays was 10 copies of H10N8 virus, and the assays did not amplify nonspecific RNA. The assays can detect H10N8 virus from chicken samples with high sensitivity and specificity, and they can serve as an effective tool for detecting and monitoring H10N8 virus in live poultry markets.

Assuntos

Vírus da Influenza A Subtipo H10N8/isolamento & purificação; Influenza Aviária/diagnóstico; Técnicas de Amplificação de Ácido Nucleico/métodos; Transcrição Reversa; Animais; Galinhas; Glicoproteínas de Hemaglutininação de Vírus da Influenza/genética; Vírus da Influenza A Subtipo H10N8/genética; Influenza Aviária/virologia; Neuraminidase/genética; Sensibilidade e Especificidade; Temperatura; Fatores de Tempo; Proteínas Virais/genética

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas de Amplificação de Ácido Nucleico / Transcrição Reversa / Vírus da Influenza A Subtipo H10N8 / Influenza Aviária Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: J Clin Microbiol Ano de publicação: 2015 Tipo de documento: Article

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