Lentivirus-Mediated Knockdown of Myosin VI Inhibits Cell Proliferation of Breast Cancer Cell.
Cancer Biother Radiopharm
; 30(8): 330-5, 2015 Oct.
Article
em En
| MEDLINE
| ID: mdl-26407123
ABSTRACT
Myosin VI (MYO6) is a unique member of the myosin superfamily, and almost no experimental studies link MYO6 to tumorigenesis of breast cancer. However, previous microarray data demonstrated that MYO6 was frequently overexpressed in breast cancer tissues. In this study, to further develop its role in breast cancer, endogenous expression of MYO6 was significantly inhibited in breast cancer ZR-75-30 and MDA-MB-231 cells using lentivirus-mediated RNA interference. Quantitative polymerase chain reaction and western blot were applied to detect the expression level of MYO6. Cell viability of both cell lines was measured by methylthiazol tetrazolium and colony formation assays. Besides, cell cycle assay was utilized to acquire the distribution information of cell phase. The results demonstrated that knockdown of MYO6 markedly reduced cell viability and colony formation, as well as suppressed cell cycle progression in breast cancer cells. The results suggested that MYO6 played a vital role in breast cancer cells and might provide useful information for diagnosis and therapy of human breast cancer in future.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
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Adenocarcinoma
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Lentivirus
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Cadeias Pesadas de Miosina
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Interferência de RNA
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Técnicas de Silenciamento de Genes
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Vetores Genéticos
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Proteínas de Neoplasias
Limite:
Female
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Humans
Idioma:
En
Revista:
Cancer Biother Radiopharm
Ano de publicação:
2015
Tipo de documento:
Article