Determination of a-glucosidase inhibitory activity from selected Fabaceae plants.

ABSTRACT

Nineteen plants from Fabaceae family, which were used in Thai traditional medicine for treatment of diabetes, were determined of α-glucosidase inhibitory activity via enzymatic reaction. In this reaction, α-glucosidase was used as enzyme, which, reacted with the substrate, p-nitrophenol-D-glucopyranoside (pNPG). After that the product, p-nitro phenol (pNP) will be occurred and observed the yellow colour at 405 nm. In this study, acarbose was used as positive standard which, inhibited this enzyme with IC50 as 331 ± 4.73 µg/ml. Caesalpinia pulcherrima leaves showed the highest activity with IC50 as 436.97 ± 9.44 µg/ml. Furthermore, Bauhinia malabarica leaves presented moderately activity with IC50 as 745.08 ± 11.15 µg/ml. However, the other plants showed mild to none activity of α-glucosidase inhibition. Accordingly, this study can support anti-diabetes of these plants in traditional medicine and it will be the database of the biological activity of Fabaceae plant.