Cloning and Characterization of 5' Flanking Regulatory Sequences of AhLEC1B Gene from Arachis Hypogaea L.
PLoS One
; 10(10): e0139213, 2015.
Article
em En
| MEDLINE
| ID: mdl-26426444
ABSTRACT
LEAFY COTYLEDON1 (LEC1) is a B subunit of Nuclear Factor Y (NF-YB) transcription factor that mainly accumulates during embryo development. We cloned the 5' flanking regulatory sequence of AhLEC1B gene, a homolog of Arabidopsis LEC1, and analyzed its regulatory elements using online software. To identify the crucial regulatory region, we generated a series of GUS expression frameworks driven by different length promoters with 5' terminal and/or 3' terminal deletion. We further characterized the GUS expression patterns in the transgenic Arabidopsis lines. Our results show that both the 65 bp proximal promoter region and the 52 bp 5' UTR of AhLEC1B contain the key motifs required for the essential promoting activity. Moreover, AhLEC1B is preferentially expressed in the embryo and is co-regulated by binding of its upstream genes with both positive and negative corresponding cis-regulatory elements.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arachis
/
Proteínas de Plantas
/
Sequências Reguladoras de Ácido Nucleico
/
Plantas Geneticamente Modificadas
/
Arabidopsis
/
Regulação da Expressão Gênica de Plantas
/
Região 5'-Flanqueadora
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
PLoS One
Ano de publicação:
2015
Tipo de documento:
Article