Bone regeneration in calvarial defects in a rat model by implantation of human bone marrow-derived mesenchymal stromal cell spheroids.
J Mater Sci Mater Med
; 26(11): 254, 2015 Nov.
Article
em En
| MEDLINE
| ID: mdl-26449444
ABSTRACT
Mesenchymal stem cell (MSC) condensation contributes to membrane ossification by enhancing their osteodifferentiation. We investigated bone regeneration in rats using the human bone marrow-derived MSC-spheroids prepared by rotation culture, without synthetic or exogenous biomaterials. Bilateral calvarial defects (8 mm) were created in nude male rats; the left-sided defects were implanted with MSC-spheroids, ß-tricalcium phosphate (ß-TCP) granules, or ß-TCP granules + MSC-spheroids, while the right-sided defects served as internal controls. Micro-computed tomography and immunohistochemical staining for osteocalcin/osteopontin indicated formation of new, full-thickness bones at the implantation sites, but not at the control sites in the MSC-spheroid group. Raman spectroscopy revealed similarity in the spectral properties of the repaired bone and native calvarial bone. Mechanical performance of the bones in the MSC-implanted group was good (50 and 60% those of native bones, respectively). All tests showed poor bone regeneration in the ß-TCP and ß-TCP + MSC-spheroid groups. Thus, significant bone regeneration was achieved with MSC-spheroid implantation into bone defects, justifying further investigation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Crânio
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Regeneração Óssea
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Células da Medula Óssea
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Transplante de Células
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Esferoides Celulares
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Modelos Animais
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Células-Tronco Mesenquimais
Limite:
Animals
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Humans
Idioma:
En
Revista:
J Mater Sci Mater Med
Ano de publicação:
2015
Tipo de documento:
Article