Labeling of membrane proteins by cell-free expression.

LaGuerre, Aisha; Löhr, Frank; Bernhard, Frank; Dötsch, Volker

LaGuerre, Aisha; Löhr, Frank; Bernhard, Frank; Dötsch, Volker.

Afiliação

LaGuerre A; Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, J.W. Goethe-University, Frankfurt-am-Main, Germany. Electronic address: laguerre@bpc.uni-frankfurt.de.
Löhr F; Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, J.W. Goethe-University, Frankfurt-am-Main, Germany.
Bernhard F; Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, J.W. Goethe-University, Frankfurt-am-Main, Germany.
Dötsch V; Institute of Biophysical Chemistry, Centre for Biomolecular Magnetic Resonance, J.W. Goethe-University, Frankfurt-am-Main, Germany.

Methods Enzymol ; 565: 367-88, 2015.

Article em En | MEDLINE | ID: mdl-26577739

Assuntos

Proteínas de Membrana/metabolismo; Sistema Livre de Células; Ressonância Magnética Nuclear Biomolecular

Palavras-chave

Amino acid scrambling; Cell lysates; Cell-free expression; Deuteration; Membrane mimetics; Membrane proteins; NMR spectroscopy; Nanodiscs; Stable isotopes; Synthetic biology

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Membrana Idioma: En Revista: Methods Enzymol Ano de publicação: 2015 Tipo de documento: Article

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