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Clinical validation of a novel real-time human papillomavirus assay for simultaneous detection of 14 high-risk HPV type and genotyping HPV type 16 and 18 in China.
Yang, Hui; Li, Lie-Jun; Xie, Long-Xu; Luo, Zhao-Yun; Lu, Min; Lin, Min; Zheng, Xiang-Bin; Huang, Yue; Yang, Li-Ye.
Afiliação
  • Yang H; Central Laboratory, Chaozhou Central Hospital Affiliated to Southern Medical University, No. 84 Huan-Cheng-Xi Road, Chaozhou, 521021, Guangdong, People's Republic of China.
  • Li LJ; Chaozhou Hybribio Limited Corporation, Chaozhou, Guangdong, People's Republic of China.
  • Xie LX; Chaozhou Hybribio Limited Corporation, Chaozhou, Guangdong, People's Republic of China.
  • Luo ZY; Central Laboratory, Chaozhou Central Hospital Affiliated to Southern Medical University, No. 84 Huan-Cheng-Xi Road, Chaozhou, 521021, Guangdong, People's Republic of China.
  • Lu M; Chaozhou Hybribio Limited Corporation, Chaozhou, Guangdong, People's Republic of China.
  • Lin M; Central Laboratory, Chaozhou Central Hospital Affiliated to Southern Medical University, No. 84 Huan-Cheng-Xi Road, Chaozhou, 521021, Guangdong, People's Republic of China.
  • Zheng XB; Central Laboratory, Chaozhou Central Hospital Affiliated to Southern Medical University, No. 84 Huan-Cheng-Xi Road, Chaozhou, 521021, Guangdong, People's Republic of China.
  • Huang Y; Central Laboratory, Chaozhou Central Hospital Affiliated to Southern Medical University, No. 84 Huan-Cheng-Xi Road, Chaozhou, 521021, Guangdong, People's Republic of China.
  • Yang LY; Central Laboratory, Chaozhou Central Hospital Affiliated to Southern Medical University, No. 84 Huan-Cheng-Xi Road, Chaozhou, 521021, Guangdong, People's Republic of China. yangleeyee@sina.com.
Arch Virol ; 161(2): 449-54, 2016 Feb.
Article em En | MEDLINE | ID: mdl-26577902
In the present study, we describe the laboratory workflow and the clinical validation of a novel multiplex real-time PCR-based HPV assay in China. The cross-sectional validation analysis showed that this assay worked well for detection of 14 HR-HPV types and identification of HPV 16 and 18 in a single sensitive assay that is suitable for both clinical usage and high-throughput cervical screening purposes. We predict that this accurate, high-throughput and low-cost HPV assay can greatly reduce the heavy economic burden of HPV detection in China.
Assuntos

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 2_ODS3 Base de dados: MEDLINE Assunto principal: Papillomaviridae / Infecções por Papillomavirus / Técnicas de Genotipagem / Reação em Cadeia da Polimerase em Tempo Real / Genótipo Tipo de estudo: Diagnostic_studies / Etiology_studies / Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Humans País/Região como assunto: Asia Idioma: En Revista: Arch Virol Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Contexto em Saúde: 2_ODS3 Base de dados: MEDLINE Assunto principal: Papillomaviridae / Infecções por Papillomavirus / Técnicas de Genotipagem / Reação em Cadeia da Polimerase em Tempo Real / Genótipo Tipo de estudo: Diagnostic_studies / Etiology_studies / Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Humans País/Região como assunto: Asia Idioma: En Revista: Arch Virol Ano de publicação: 2016 Tipo de documento: Article