Pyrrole-Imidazole Polyamides: Manual Solid-Phase Synthesis.
Curr Protoc Nucleic Acid Chem
; 63: 8.10.1-8.10.41, 2015 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-26623975
ABSTRACT
Pyrrole-imidazole polyamides (PAs) are a family of DNA-binding peptides that bind in the minor groove of double-stranded DNA (dsDNA) in a sequence-selective, programmable fashion. This protocol describes a detailed manual procedure for the solid-phase synthesis of this family of compounds. The protocol entails solution-phase synthesis of the Boc-protected pyrrole (Py) and imidazole (Im) carboxylic acid building blocks. This unit also describes the importance of choosing the appropriate condensing agent to form the amide linkages between each building block. Finally, a monomeric coupling protocol and a fragment-based approach are described that delivers PAs in 13% to 30% yield in 8 days.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poliaminas
/
Pirróis
/
Imidazóis
Idioma:
En
Revista:
Curr Protoc Nucleic Acid Chem
Ano de publicação:
2015
Tipo de documento:
Article