Assay development for determination of tenofovir in human plasma by solid phase analytical derivatization and LC-MS/MS.
Bioanalysis
; 7(24): 3085-95, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-26626536
ABSTRACT
BACKGROUND:
A novel method was developed and validated to measure tenofovir in human plasma. RESULTS/METHODOLOGY:
This method employed solid phase analytical derivatization and analysis by LC-MS/MS. Stable-labeled internal standard was added to plasma samples followed by solid phase extraction. Retained analytes were derivatized on the solid phase extraction cartridges with a diazomethane solution to yield methyl-ester derivatives. Samples were analyzed using LC-MS/MS incorporating the use of a strong cation exchange column. The method was validated over a range of 5.00-750 ng/ml. The approach developed in this report for tenofovir could be applied to other analytes that share similar structural similarities.CONCLUSION:
The tenofovir LC-MS/MS method was used to support a clinical study of over 400 samples with a 100% success rate.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Antivirais
/
Cromatografia Líquida
/
Espectrometria de Massas em Tandem
/
Extração em Fase Sólida
/
Tenofovir
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2015
Tipo de documento:
Article