Detection by immunogold techniques of HIV antigens in Lowicryl ultrathin sections of infected cells.
Pathol Res Pract
; 184(5): 494-7, 1989 May.
Article
em En
| MEDLINE
| ID: mdl-2664735
ABSTRACT
A post-embedding technique for immunocytochemical analysis at the ultrastructural level was used to detect and localize HIV antigens on ultrathin sections of Lowicryl K4M-embedded HIV-infected cells. With serum from an AIDS patient, specific immunogold labelling was obtained exclusively on mature viral extracellular structures. The more intense reactivity was obtained with core antigens. The present immunoelectron microscopy method provides several advantages - high sensitivity of immunodetection, good preservation of cellular morphology, easy preparation procedure - which could lead to the use of this method for HIV-infected human tissues.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Resinas Acrílicas
/
Antígenos HIV
/
Técnicas Histológicas
/
Técnicas Imunológicas
/
Síndrome da Imunodeficiência Adquirida
/
Ouro
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Pathol Res Pract
Ano de publicação:
1989
Tipo de documento:
Article