In vitro effects of deoxynivalenol and zearalenone major metabolites alone and combined, on cell proliferation, steroid production and gene expression in bovine small-follicle granulosa cells.
Toxicon
; 109: 70-83, 2016 Jan.
Article
em En
| MEDLINE
| ID: mdl-26657070
ABSTRACT
The effects of deoxynivalenol (DON) and zearalenone (ZEA) on reproduction in ruminants are unclear. This study was performed to evaluate the impact of DON and ZEA hydroxylated metabolites, α-zearalenol (α-Zol) and ß-zearalenone (ß-Zol), on cell proliferation, steroidogenesis and gene expression using bovine granulosa cells (GC). Cell proliferation was negatively affected after exposure to ß-Zol at 31 µM and after exposure to α-Zol (3.1 µM) alone and combined with DON (3.3 µM). DON and α-Zol decreased steroidogenesis, while ß-Zol at high concentration had stimulatory effects. DON and ß-Zol increased CYP19A1 mRNA abundance. CYP11A1 mRNA abundance was stimulated by DON, alone and combined with α-Zol and ß-Zol, whereas was inhibited by ß-Zol alone. Generally mycotoxins effects on cell proliferation, steroidogenesis and gene expression were influenced by the presence or absence of IGF1. In conclusion DON and ZEA metabolites may impair in vitro cell proliferation, steroid production and gene expression in cattle.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esteroides
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Tricotecenos
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Zearalenona
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Expressão Gênica
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Proliferação de Células
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Células da Granulosa
Limite:
Animals
Idioma:
En
Revista:
Toxicon
Ano de publicação:
2016
Tipo de documento:
Article