Nutrient control for stationary phase cellulase production in Trichoderma reesei Rut C-30.
Enzyme Microb Technol
; 82: 8-14, 2016 Jan.
Article
em En
| MEDLINE
| ID: mdl-26672443
This work describes the use of nutrient limitations with Trichoderma reesei Rut C-30 to obtain a prolonged stationary phase cellulase production. This period of non-growth may allow for dependable cellulase production, extended fermentation periods, and the possibility to use pellet morphology for easy product separation. Phosphorus limitation was successful in halting growth and had a corresponding specific cellulase production of 5±2 FPU/g-h. Combined with the addition of Triton X-100 for fungal pellet formation and low shear conditions, a stationary phase cellulase production period in excess of 300 h was achieved, with a constant enzyme production rate of 7±1 FPU/g-h. While nitrogen limitation was also effective as a growth limiter, it, however, also prevented cellulase production.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fósforo
/
Trichoderma
/
Proteínas Fúngicas
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Celulase
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Meios de Cultura
/
Micologia
/
Nitrogênio
Tipo de estudo:
Evaluation_studies
Idioma:
En
Revista:
Enzyme Microb Technol
Ano de publicação:
2016
Tipo de documento:
Article