A comb-like branched ß-D-glucan produced by a Cordyceps sinensis fungus and its protective effect against cyclophosphamide-induced immunosuppression in mice.
Carbohydr Polym
; 142: 259-67, 2016 May 20.
Article
em En
| MEDLINE
| ID: mdl-26917398
ABSTRACT
An exopolysaccharide (EPS) was fractionated from fermentation media of a Cordyceps sinensis fungus (Cs-HK1) by ethanol precipitation at 2/5 volume ratio of ethanol/media. Its structural characteristics were elucidated by FT-IR, GC, GC-MS, 1D and 2D NMR combined with periodate oxidation, Smith degradation, partial acid hydrolysis, and methylation analysis. Furthermore, the immunomodulatory activity of EPS was evaluated by the model of cyclophosphamide-induced immunosuppression. The results from monosaccharide composition and partial acid hydrolysis indicated that EPS almost consisted of glucose excluding a trace amount of mannose. GC-MS and NMR analysis further confirmed EPS had a linear backbone of (1â3)-ß-D-glucopyranosyl residues with a single (1â6)-ß-D-glucopyranosyl side-branching unit for every three ß-D-glucopyranosyl residues, showing a comb-like ß-D-glucan with short and intensive branches, which was responsible for high viscosity. Moreover, EPS could significantly enhance immune organs and stimulate the release of major cytokines TNF-α and INF-γ, suggesting that EPS exhibited protective effect in immunocompromised mice.
Palavras-chave
Acetic anhydride (PubChem CID: 7918); Anthrone (PubChem CID: 7018); Chloroform (PubChem CID: 6212); Comb-like branched structure; Cordyceps sinensis; Cyclophosphamide-induced immunosuppression; DMSO-d(6) (PubChem CID: 75151); Dimethyl sulfoxide (PubChem CID: 679); Ethylene glycol (PubChem CID: 174); Formic acid (PubChem CID: 284); Hydroxylamine hydrochloride (PubChem CID: 443297); Methyl iodide (PubChem CID: 6328); Protective effect; Pyridine (PubChem CID: 1049); Sodium hydroxide (PubChem CID: 14798); Sodium periodate (PubChem CID: 23667635); Trifluoroacetic acid (PubChem CID: 6422); d-Arabinose (PubChem CID: 229); d-Fucose (PubChem CID: 840); d-Galactose (PubChem CID: 6036); d-Glucose (PubChem CID: 5793); d-Mannose (PubChem CID: 18950); d-Rhamnose (PubChem CID: 5460029); d-Xylose (PubChem CID: 135191); ß-d-Glucan
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ciclofosfamida
/
Cordyceps
/
Beta-Glucanas
/
Fatores Imunológicos
/
Imunossupressores
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2016
Tipo de documento:
Article