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Detection of the Antimicrobial Triclosan in Environmental Samples by Immunoassay.
Ahn, Ki Chang; Ranganathan, Anupama; Bever, Candace S; Hwang, Sung Hee; Holland, Erika B; Morisseau, Kevin; Pessah, Isaac N; Hammock, Bruce D; Gee, Shirley J.
Afiliação
  • Ahn KC; Department of Entomology and Nematology and UCD Comprehensive Cancer Center and ‡Department of Molecular Biosciences, University of California Davis , Davis, California 95616, United States.
  • Ranganathan A; Department of Entomology and Nematology and UCD Comprehensive Cancer Center and ‡Department of Molecular Biosciences, University of California Davis , Davis, California 95616, United States.
  • Bever CS; Department of Entomology and Nematology and UCD Comprehensive Cancer Center and ‡Department of Molecular Biosciences, University of California Davis , Davis, California 95616, United States.
  • Hwang SH; Department of Entomology and Nematology and UCD Comprehensive Cancer Center and ‡Department of Molecular Biosciences, University of California Davis , Davis, California 95616, United States.
  • Holland EB; Department of Entomology and Nematology and UCD Comprehensive Cancer Center and ‡Department of Molecular Biosciences, University of California Davis , Davis, California 95616, United States.
  • Morisseau K; Department of Entomology and Nematology and UCD Comprehensive Cancer Center and ‡Department of Molecular Biosciences, University of California Davis , Davis, California 95616, United States.
  • Pessah IN; Department of Entomology and Nematology and UCD Comprehensive Cancer Center and ‡Department of Molecular Biosciences, University of California Davis , Davis, California 95616, United States.
  • Hammock BD; Department of Entomology and Nematology and UCD Comprehensive Cancer Center and ‡Department of Molecular Biosciences, University of California Davis , Davis, California 95616, United States.
  • Gee SJ; Department of Entomology and Nematology and UCD Comprehensive Cancer Center and ‡Department of Molecular Biosciences, University of California Davis , Davis, California 95616, United States.
Environ Sci Technol ; 50(7): 3754-61, 2016 Apr 05.
Article em En | MEDLINE | ID: mdl-26937944
ABSTRACT
A sensitive, competitive enzyme-linked immunosorbent assay (ELISA) for the detection of the antimicrobial triclosan (TCS; 2,4,4'-trichloro-2'-hydroxydiphenyl ether) was developed. Novel immunizing haptens were synthesized by derivatizing at the 4-Cl position of the TCS molecule. Compounds derived from substitutions at 4'-Cl and that replaced the 2'-OH with a Cl atom were designed as unique coating antigen haptens. Polyclonal rabbit antisera were screened against the coating antigen library to identify combinations of immunoreagents resulting in the most sensitive assays. The most sensitive assay identified was one utilizing antiserum no. 1155 and a heterologous competitive hapten, where the 2'-OH group was substituted with a Cl atom. An IC50 value and the detection range for TCS in assay buffer were 1.19 and 0.21-6.71 µg/L, respectively. The assay was selective for TCS, providing low cross-reactivity (<5%) to the major metabolites of TCS and to brominated diphenyl ether-47. A second assay utilizing a competitive hapten containing Br instead of Cl substitutions was broadly selective for both brominated and chlorinated diphenylethers. Using the most sensitive assay combination, we measured TCS concentrations in water samples following dilution. Biosolid samples were analyzed following the dilution of a simple solvent extract. The immunoassay results were similar to those determined by LC-MS/MS. This immunoassay can be used as a rapid and convenient tool to screen for human and environmental exposure.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Triclosan / Poluentes Químicos da Água / Ensaio de Imunoadsorção Enzimática / Anti-Infecciosos Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Environ Sci Technol Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Triclosan / Poluentes Químicos da Água / Ensaio de Imunoadsorção Enzimática / Anti-Infecciosos Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Environ Sci Technol Ano de publicação: 2016 Tipo de documento: Article