A Preclinical Model for ERα-Positive Breast Cancer Points to the Epithelial Microenvironment as Determinant of Luminal Phenotype and Hormone Response.
Cancer Cell
; 29(3): 407-422, 2016 Mar 14.
Article
em En
| MEDLINE
| ID: mdl-26947176
ABSTRACT
Seventy-five percent of breast cancers are estrogen receptor α positive (ERâº). Research on these tumors is hampered by lack of adequate in vivo models; cell line xenografts require non-physiological hormone supplements, and patient-derived xenografts (PDXs) are hard to establish. We show that the traditional grafting of ER⺠tumor cells into mammary fat pads induces TGFß/SLUG signaling and basal differentiation when they require low SLUG levels to grow in vivo. Grafting into the milk ducts suppresses SLUG; ER⺠tumor cells develop, like their clinical counterparts, in the presence of physiological hormone levels. Intraductal ER⺠PDXs are retransplantable, predictive, and appear genomically stable. The model provides opportunities for translational research and the study of physiologically relevant hormone action in breast carcinogenesis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
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Glândulas Mamárias Humanas
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Receptor alfa de Estrogênio
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Microambiente Tumoral
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
Cancer Cell
Ano de publicação:
2016
Tipo de documento:
Article