An unbiased metric of antiproliferative drug effect in vitro.
Nat Methods
; 13(6): 497-500, 2016 06.
Article
em En
| MEDLINE
| ID: mdl-27135974
ABSTRACT
In vitro cell proliferation assays are widely used in pharmacology, molecular biology, and drug discovery. Using theoretical modeling and experimentation, we show that current metrics of antiproliferative small molecule effect suffer from time-dependent bias, leading to inaccurate assessments of parameters such as drug potency and efficacy. We propose the drug-induced proliferation (DIP) rate, the slope of the line on a plot of cell population doublings versus time, as an alternative, time-independent metric.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proliferação de Células
/
Bibliotecas de Moléculas Pequenas
/
Descoberta de Drogas
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Modelos Teóricos
/
Biologia Molecular
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Nat Methods
Ano de publicação:
2016
Tipo de documento:
Article