Bayesian methods for event analysis of intracellular currents.
J Neurosci Methods
; 269: 21-32, 2016 08 30.
Article
em En
| MEDLINE
| ID: mdl-27208694
ABSTRACT
BACKGROUND:
Investigation of neural circuit functioning often requires statistical interpretation of events in subthreshold electrophysiological recordings. This problem is non-trivial because recordings may have moderate levels of structured noise and events may have distinct kinetics. In addition, novel experimental designs that combine optical and electrophysiological methods will depend upon statistical tools that combine multimodal data. NEWMETHOD:
We present a Bayesian approach for inferring the timing, strength, and kinetics of post-synaptic currents (PSCs) from voltage-clamp electrophysiological recordings on a per event basis. The simple generative model for a single voltage-clamp recording flexibly extends to include additional structure to enable experiments designed to probe synaptic connectivity.RESULTS:
We validate the approach on simulated and real data. We also demonstrate that extensions of the basic PSC detection algorithm can handle recordings contaminated with optically evoked currents, and we simulate a scenario in which calcium imaging observations, available for a subset of neurons, can be fused with electrophysiological data to achieve higher temporal resolution. COMPARISON WITH EXISTINGMETHODS:
We apply this approach to simulated and real ground truth data to demonstrate its higher sensitivity in detecting small signal-to-noise events and its increased robustness to noise compared to standard methods for detecting PSCs.CONCLUSIONS:
The new Bayesian event analysis approach for electrophysiological recordings should allow for better estimation of physiological parameters under more variable conditions and help support new experimental designs for circuit mapping.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sinapses
/
Algoritmos
/
Reconhecimento Automatizado de Padrão
/
Técnicas de Patch-Clamp
/
Potenciais Sinápticos
/
Automação Laboratorial
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Neurosci Methods
Ano de publicação:
2016
Tipo de documento:
Article