Alcoholic Steatosis in Different Strains of Rat: A Comparative Study.
J Drug Alcohol Res
; 42015.
Article
em En
| MEDLINE
| ID: mdl-27213081
ABSTRACT
BACKGROUND:
Different strains of rats have been used to study alcoholic liver disease (ALD) while the reason for selecting a particular rat strain was not apparent.PURPOSE:
The aim of our study was to compare outbred (Wistar) and inbred (Fischer) strains to evaluate pathological, biochemical changes, and gene expression differences associated with ethanol-induced early hepatic steatosis. STUDYDESIGN:
Male Wistar and Fischer-344 rats were pair-fed for 6 weeks with or without 5% ethanol in Lieber-DeCarli liquid diet. Livers were analyzed for histological and lipid-related differences.RESULTS:
Hepatic midzonal steatosis was mainly found in Wistar rats while Fischer rats showed mostly pericentral steatosis. Increased hepatic steatosis in ethanol-fed Wistar rats is supported by increases in lipids with related genes and transcription factors involved in fatty acid and triglyceride synthesis.CONCLUSION:
Our data showed that Fischer rats are relatively less prone to ethanol-mediated steatosis with pericentral lipid deposition pattern in the liver which is similar to humans and show no trace level of lipid accumulation in pair-fed controls as observed in Wistar (outbred) strain. Therefore, Fischer rats are better suited for lipid studies in an early development of ALD.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
J Drug Alcohol Res
Ano de publicação:
2015
Tipo de documento:
Article