Development of a highly sensitive and specific monoclonal antibody based enzyme-linked immunosorbent assay for the detection of a new ß-agonist, phenylethanolamine A, in food samples.
J Sci Food Agric
; 97(3): 1001-1009, 2017 Feb.
Article
em En
| MEDLINE
| ID: mdl-27247162
ABSTRACT
BACKGROUND:
All ß-agonists are banned as feed additives for growth promotion in animals due to toxic effects on humans after consuming the ß-agonist contaminated meats. Phenylethanolamine A (PA) is a newly emerged ß-agonist. Thus there is a need to develop highly sensitive and specific analytical methods for the detection of PA in food samples. In this study, the monoclonal antibody (mAb) against PA was produced by hybridoma technology and used for the development of enzyme-linked immunosorbent assay (ELISA).RESULTS:
The IC50 values and limits of detection (LODs) of the ELISA using homogeneous combination of coating antigen/antibody for PA were 0.16 ng mL-1 and 0.011 ng mL-1 , respectively. The cross-reactive (CR) values of the assay with 14 structurally related ß-agonists were lower than 0.59%. Swine liver and meat samples were spiked with PA at different content and analysed by ELISA. Acceptable recovery rates of 91.40-105.51% and intra-assay coefficients of variation of 1.56-9.92% (n = 3) were obtained. The ELISA for seven spiked samples was confirmed by LC-MS/MS with a high correlation coefficient of 0.9881.CONCLUSION:
The proposed mAb-based ELISA was highly sensitive and specific for PA and could be used as a quantitative/screening method for PA analysis in food samples. © 2016 Society of Chemical Industry.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
2_ODS3
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
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Resíduos de Drogas
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2-Hidroxifenetilamina
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Inspeção de Alimentos
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Agonistas Adrenérgicos beta
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Anticorpos Monoclonais
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Especificidade de Anticorpos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
J Sci Food Agric
Ano de publicação:
2017
Tipo de documento:
Article