Drosophila cells use nanotube-like structures to transfer dsRNA and RNAi machinery between cells.
Sci Rep
; 6: 27085, 2016 06 03.
Article
em En
| MEDLINE
| ID: mdl-27255932
ABSTRACT
Tunnelling nanotubes and cytonemes function as highways for the transport of organelles, cytosolic and membrane-bound molecules, and pathogens between cells. During viral infection in the model organism Drosophila melanogaster, a systemic RNAi antiviral response is established presumably through the transport of a silencing signal from one cell to another via an unknown mechanism. Because of their role in cell-cell communication, we investigated whether nanotube-like structures could be a mediator of the silencing signal. Here, we describe for the first time in the context of a viral infection the presence of nanotube-like structures in different Drosophila cell types. These tubules, made of actin and tubulin, were associated with components of the RNAi machinery, including Argonaute 2, double-stranded RNA, and CG4572. Moreover, they were more abundant during viral, but not bacterial, infection. Super resolution structured illumination microscopy showed that Argonaute 2 and tubulin reside inside the tubules. We propose that nanotube-like structures are one of the mechanisms by which Argonaute 2, as part of the antiviral RNAi machinery, is transported between infected and non-infected cells to trigger systemic antiviral immunity in Drosophila.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
RNA de Cadeia Dupla
/
Organelas
/
Proteínas de Drosophila
/
Drosophila melanogaster
/
Proteínas Argonautas
Idioma:
En
Revista:
Sci Rep
Ano de publicação:
2016
Tipo de documento:
Article