Production of 4-androstene-3,17-dione and 1,4-androstadiene-3,17-dione from rice germ and wheat germ extracts by Mycobacterium sp.
Biotechnol Lett
; 38(9): 1595-602, 2016 Sep.
Article
em En
| MEDLINE
| ID: mdl-27262293
ABSTRACT
OBJECTIVE:
To study the biotransformation of phytosterol and phytosterol-containing rice germ and wheat germ ethanolic extracts to produce 4-androstene-3,17-dione (AD) and 1,4-androstadiene-3,17-dione (ADD) by Mycobacterium sp. DSM 2966 using phytosterol to hydroxypropyl-ß-cyclodextrin (21, 11 and 12 mol/mol) and 2 % (w/v) Tween 80 as solubilizing agents.RESULTS:
A maximum yield of 180 ± 27 mg AD l(-1) and 31 ± 11.4 mg ADD l(-1) with a total conversion of 65 % (day 12) was obtained using 1 g phytosterol l(-1) and hydroxypropyl-ß-cyclodextrin (2ââ1 mol/mol) with 2 % (w/v) Tween 80 in the fermentation medium. The most appropriate conditions for rice germ extract and wheat germ extract which gave the maximum conversion of 22 and 43 % (day 14) were obtained by using 2 % (w/v) Tween 80.CONCLUSIONS:
Phytosterol and wheat germ are effective sources for AD and ADD production while rice germ required further development. Hydroxypropyl-ß-cyclodextrin (2â1 mol/mol) and/or 2 % (w/v) Tween 80 in the biotransformation process could improve AD and ADD yields, depending on substrates and biotransformation conditions.Palavras-chave
Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oryza
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Triticum
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Extratos Vegetais
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Androstadienos
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Androstenodiona
/
Mycobacterium
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2016
Tipo de documento:
Article