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High molecular weight of polysaccharides from Hericium erinaceus against amyloid beta-induced neurotoxicity.
Cheng, Jai-Hong; Tsai, Chia-Ling; Lien, Yi-Yang; Lee, Meng-Shiou; Sheu, Shyang-Chwen.
Afiliação
  • Cheng JH; Department of Medical Research, Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.
  • Tsai CL; Department of Food Science, National Pingtung University of Science and Technology, No. 1, Shuehfu Rd, Neipu, Pingtung, Taiwan.
  • Lien YY; Department of Veterinary Medicine, National Pingtung University of Science and Technology, No. 1, Shuehfu Rd, Neipu, Pingtung, Taiwan.
  • Lee MS; Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan. leemengshiou@mail.cmu.edu.tw.
  • Sheu SC; Department of Food Science, National Pingtung University of Science and Technology, No. 1, Shuehfu Rd, Neipu, Pingtung, Taiwan. ssheu@mail.npust.edu.tw.
BMC Complement Altern Med ; 16: 170, 2016 Jun 07.
Article em En | MEDLINE | ID: mdl-27266872
BACKGROUND: Hericium erinaceus (HE) is a well-known mushroom in traditional Chinese food and medicine. HE extracts from the fruiting body and mycelia not only exhibit immunomodulatory, antimutagenic and antitumor activity but also have neuroprotective properties. Here, we purified HE polysaccharides (HEPS), composed of two high molecular weight polysaccharides (1.7 × 10(5) Da and 1.1 × 10(5) Da), and evaluated their protective effects on amyloid beta (Aß)-induced neurotoxicity in rat pheochromocytoma PC12 cells. METHODS: HEPS were prepared and purified using a 95 % ethanol extraction method. The components of HEPS were analyzed and the molecular weights of the polysaccharides were determined using high-pressure liquid chromatography (HPLC). The neuroprotective effects of the polysaccharides were evaluated through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and an MTT assay and by quantifying reactive oxygen species (ROS) and mitochondrial membrane potentials (MMP) of Aß-induced neurotoxicity in cells. RESULT: Our results showed that 250 µg/ml HEPS was harmless and promoted cell viability with 1.2 µM Aß treatment. We observed that the free radical scavenging rate exceeded 90 % when the concentration of HEPS was higher than 1 mg/mL in cells. The HEPS decreased the production of ROS from 80 to 58 % in a dose-dependent manner. Cell pretreatment with 250 µg/mL HEPS significantly reduced Aß-induced high MMPs from 74 to 51 % and 94 to 62 % at 24 and 48 h, respectively. Finally, 250 µg/mL of HEPS prevented Aß-induced cell shrinkage and nuclear degradation of PC12 cells. CONCLUSION: Our results demonstrate that HEPS exhibit antioxidant and neuroprotective effects on Aß-induced neurotoxicity in neurons.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polissacarídeos / Basidiomycota / Fármacos Neuroprotetores Limite: Animals Idioma: En Revista: BMC Complement Altern Med Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polissacarídeos / Basidiomycota / Fármacos Neuroprotetores Limite: Animals Idioma: En Revista: BMC Complement Altern Med Ano de publicação: 2016 Tipo de documento: Article