Nitration is exclusive to defense-related PR-1, PR-3 and PR-5 proteins in tobacco leaves.
Plant Signal Behav
; 11(7): e1197464, 2016 07 02.
Article
em En
| MEDLINE
| ID: mdl-27301959
ABSTRACT
Protein tyrosine nitration is an important post-translational modification. A variety of nitrated proteins are reported in Arabidopsis leaves and seedlings, sunflower hypocotyls, and pea roots. The identities of nitrated proteins are species-/organ-specific, and chloroplast proteins are most nitratable in leaves. However, precise mechanism is unclear. Here, we investigated nitroproteome in tobacco leaves following exposure to nitrogen dioxide. Proteins were extracted, electrophoresed and immunoblotted using an anti-3-nitrotyrosine antibody. Mass spectrometry and FASTA search identified for the first time an exclusive nitration of pathogenesis-related proteins, PR-1, PR-3 and PR-5, which are reportedly located in the apoplast or the vacuole. Furthermore, Tyr(36) of thaumatin-like protein E2 was identfied as a nitration site. The underlying mechanism and physiological relevance are discussed.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Plantas
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Nicotiana
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Folhas de Planta
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Nitratos
Idioma:
En
Revista:
Plant Signal Behav
Ano de publicação:
2016
Tipo de documento:
Article