Detection of HIV-specific antibodies in infancy by isoelectric focusing and affinity immunoblotting.
J Infect Dis
; 160(1): 126-30, 1989 Jul.
Article
em En
| MEDLINE
| ID: mdl-2732508
ABSTRACT
Asymptomatic congenital HIV infection cannot be diagnosed in infants less than 15 mo by routine serologic techniques because of the presence of passively acquired maternal antibody in the infants' circulation. Possibly, infants who synthesize antibody to highly conserved HIV proteins may be recognized by the detection in serum of clonally distinct IgG antibodies to HIV. To test this hypothesis, isoelectric focusing in thin-layer agarose gels was combined with affinity immunoblotting to antigen-coated nitrocellulose membranes. In all 10 cases examined, the presence or absence of clonotypically distinct bands of IgG antibodies was concordant with infectious status. Thus, this technique may provide an accurate serologic approach to the diagnosis of congenital HIV infection.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Anticorpos Anti-HIV
/
Immunoblotting
/
Síndrome da Imunodeficiência Adquirida
/
Focalização Isoelétrica
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
/
Humans
/
Infant
/
Newborn
Idioma:
En
Revista:
J Infect Dis
Ano de publicação:
1989
Tipo de documento:
Article