The positive transcriptional elongation factor (P-TEFb) is required for neural crest specification.
Dev Biol
; 416(2): 361-72, 2016 08 15.
Article
em En
| MEDLINE
| ID: mdl-27343897
ABSTRACT
Regulation of gene expression at the level of transcriptional elongation has been shown to be important in stem cells and tumour cells, but its role in the whole animal is only now being fully explored. Neural crest cells (NCCs) are a multipotent population of cells that migrate during early development from the dorsal neural tube throughout the embryo where they differentiate into a variety of cell types including pigment cells, cranio-facial skeleton and sensory neurons. Specification of NCCs is both spatially and temporally regulated during embryonic development. Here we show that components of the transcriptional elongation regulatory machinery, CDK9 and CYCLINT1 of the P-TEFb complex, are required to regulate neural crest specification. In particular, we show that expression of the proto-oncogene c-Myc and c-Myc responsive genes are affected. Our data suggest that P-TEFb is crucial to drive expression of c-Myc, which acts as a 'gate-keeper' for the correct temporal and spatial development of the neural crest.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Xenopus laevis
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Genes myc
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Regulação da Expressão Gênica no Desenvolvimento
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Proteínas de Xenopus
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Quinase 9 Dependente de Ciclina
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Fator B de Elongação Transcricional Positiva
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Ciclina T
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Elongação da Transcrição Genética
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Crista Neural
Limite:
Animals
Idioma:
En
Revista:
Dev Biol
Ano de publicação:
2016
Tipo de documento:
Article