Molecular cloning and expression analysis of a new lily-type lectin in the rock bream, Oplegnathus fasciatus.
Dev Comp Immunol
; 65: 25-30, 2016 12.
Article
em En
| MEDLINE
| ID: mdl-27345170
ABSTRACT
A new lily-type lectin RbLTL was identified from rock bream (Oplegnathus fasciatus) and its expression analysed. In this study, a new lily-type lectin gene (RbLTL) was cloned from rock bream using expressed sequence tag (EST) analysis. The full-length RbLTL cDNA was encoding a 117-amino acid protein. The deduced amino acid sequence of RbLTL contained all of the conserved features crucial for its fundamental structure, including B-lectin domain and three d-mannose binding sites. RbLTL mRNA was predominately expressed in the gills, with reduced expression noted in intestine tissue. Expression analysis of time series sampled fertilized eggs revealed that expression gradually increased 1, 3, 12, and 24 h However, expression decreased at 36 h. RbLTL expression was differentially up-regulated in rock bream gills challenged with Streptococcus iniae, Edwardsiella tarda and RSIV. Our results revealed that novel rock bream lily-type lectin may be an important molecule involved in pattern recognition and pathogen elimination in the innate immunity of rock bream.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Infecções Estreptocócicas
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Zigoto
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Perciformes
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Edwardsiella tarda
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Proteínas de Peixes
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Lectinas de Ligação a Manose
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Infecções por Vírus de DNA
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Infecções por Enterobacteriaceae
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Streptococcus iniae
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Brânquias
Limite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Ano de publicação:
2016
Tipo de documento:
Article