Comparison of TaqMan(®) Array Card and MYCOTB(TM) with conventional phenotypic susceptibility testing in MDR-TB.
Int J Tuberc Lung Dis
; 20(8): 1105-12, 2016 08.
Article
em En
| MEDLINE
| ID: mdl-27393547
ABSTRACT
BACKGROUND:
Although phenotypic drug susceptibility testing (DST) is endorsed as the standard for second-line drug testing of Mycobacterium tuberculosis, it is slow and laborious.METHODS:
We evaluated the accuracy of two faster, easier methodologies that provide results for multiple drugs a genotypic TaqMan(®) Array Card (TAC) and the Sensititre(®) MYCOTB(TM) plate. Both methods were tested at three central laboratories in Bangladesh, Tanzania, and Thailand with 212 multidrug-resistant tuberculosis (MDR-TB) isolates and compared with the laboratories' phenotypic method in use.RESULTS:
The overall accuracy for ethambutol, streptomycin, amikacin, kanamycin, ofloxacin, and moxifloxacin vs. the phenotypic standard was 87% for TAC (range 70-99) and 88% for the MYCOTB plate (range 76-98). To adjudicate discordances, we re-defined the standard as the consensus of the three methods, against which the TAC and MYCOTB plate yielded 94-95% accuracy, while the phenotypic result yielded 93%. Some isolates with genotypic mutations and high minimum inhibitory concentration (MIC) were phenotypically susceptible, and some isolates without mutations and low MIC were phenotypically resistant, questioning the phenotypic standard.CONCLUSIONS:
In our view, the TAC, the MYCOTB plate, and the conventional phenotypic method have similar performance for second-line drugs; however, the former methods offer speed, throughput, and quantitative DST information.
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Análise Mutacional de DNA
/
Tuberculose Resistente a Múltiplos Medicamentos
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Reação em Cadeia da Polimerase em Tempo Real
/
Mutação
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Mycobacterium tuberculosis
Tipo de estudo:
Clinical_trials
/
Evaluation_studies
/
Prognostic_studies
Limite:
Humans
País/Região como assunto:
Africa
/
Asia
Idioma:
En
Revista:
Int J Tuberc Lung Dis
Ano de publicação:
2016
Tipo de documento:
Article