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Multicenter validation of CSF neurofilaments as diagnostic biomarkers for ALS.
Oeckl, Patrick; Jardel, Claude; Salachas, François; Lamari, Foudil; Andersen, Peter M; Bowser, Robert; de Carvalho, Mamede; Costa, Júlia; van Damme, Philip; Gray, Elizabeth; Grosskreutz, Julian; Hernández-Barral, María; Herukka, Sanna-Kaisa; Huss, André; Jeromin, Andreas; Kirby, Janine; Kuzma-Kozakiewicz, Magdalena; Amador, Maria Del Mar; Mora, Jesús S; Morelli, Claudia; Muckova, Petra; Petri, Susanne; Poesen, Koen; Rhode, Heidrun; Rikardsson, Anna-Karin; Robberecht, Wim; Rodríguez Mahillo, Ana I; Shaw, Pamela; Silani, Vincenzo; Steinacker, Petra; Turner, Martin R; Tüzün, Erdem; Yetimler, Berrak; Ludolph, Albert C; Otto, Markus.
Afiliação
  • Oeckl P; a Department of Neurology , Ulm University Hospital , 89081 Ulm , Germany ;
  • Jardel C; b Department of Metabolic Biochemistry , Hôpitaux universitaires Pitié Salpétrière-Charles Foix , 75651 Paris , France .
  • Salachas F; c Paris ALS Reference Center, Neurological Diseases Department , Hôpitaux universitaires La Pitié Salpêtrière-Charles Foix , 75651 Paris , France .
  • Lamari F; b Department of Metabolic Biochemistry , Hôpitaux universitaires Pitié Salpétrière-Charles Foix , 75651 Paris , France .
  • Andersen PM; d Department of Neurology , University of Umea , 90185 Umea , Sweden .
  • Bowser R; e Iron Horse Diagnostics, Inc. , 85255 Scottsdale , Arizona , USA .
  • de Carvalho M; f Divisions of Neurology and Neurobiology , Barrow Neurological Institute , 85013 Phoenix , Arizona , USA .
  • Costa J; g Faculty of Medicine - Instituto de Medicina Molecular , University of Lisbon , 1649-028 Lisbon , Portugal .
  • van Damme P; h Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa , 2780-157 Oeiras , Portugal .
  • Gray E; i University Hospitals Leuven , Department of Neurology , 3000 Leuven , Belgium .
  • Grosskreutz J; s KU Leuven - University of Leuven , Department of Neurosciences, VIB - Vesalius Research Center, Experimental Neurology - Laboratory of Neurobiology , Leuven , Belgium .
  • Hernández-Barral M; j Nuffield Department of Clinical Neurosciences , University of Oxford , OX3 9DU Oxford , UK .
  • Herukka SK; k Department of Neurology , Jena University Hospital , 07747 Jena , Germany .
  • Huss A; t ALS Unit, Hospital Carlos III, Madrid , 28029 Madrid , Spain .
  • Jeromin A; l Department of Neurology , University of Eastern Finland and Kuopio University Hospital , 70211 Kuopio , Finland .
  • Kirby J; a Department of Neurology , Ulm University Hospital , 89081 Ulm , Germany ;
  • Kuzma-Kozakiewicz M; e Iron Horse Diagnostics, Inc. , 85255 Scottsdale , Arizona , USA .
  • Amador Mdel M; m Department of Neuroscience , Sheffield Institute for Translational Neuroscience, University of Sheffield , S10 2HQ Sheffield , UK .
  • Mora JS; n Department of Neurology , Medical University of Warsaw , 02-097 Warsaw , Poland .
  • Morelli C; c Paris ALS Reference Center, Neurological Diseases Department , Hôpitaux universitaires La Pitié Salpêtrière-Charles Foix , 75651 Paris , France .
  • Muckova P; t ALS Unit, Hospital Carlos III, Madrid , 28029 Madrid , Spain .
  • Petri S; o IRCCS Istituto Auxologico Italiano, Department of Neurology and Laboratory of Neuroscience, 20149 Milano, Italy .
  • Poesen K; p Institute of Biochemistry I, Jena University Hospital , 07743 Jena , Germany .
  • Rhode H; q Department of Neurology , Hannover Medical School , 30625 Hannover , Germany .
  • Rikardsson AK; r Laboratory of molecular neurobiomarker research , University of Leuven and Laboratory Medicine, University Hospitals of Leuven , 3000 Leuven , Belgium .
  • Robberecht W; p Institute of Biochemistry I, Jena University Hospital , 07743 Jena , Germany .
  • Rodríguez Mahillo AI; d Department of Neurology , University of Umea , 90185 Umea , Sweden .
  • Shaw P; s KU Leuven - University of Leuven , Department of Neurosciences, VIB - Vesalius Research Center, Experimental Neurology - Laboratory of Neurobiology , Leuven , Belgium .
  • Silani V; t ALS Unit, Hospital Carlos III, Madrid , 28029 Madrid , Spain .
  • Steinacker P; m Department of Neuroscience , Sheffield Institute for Translational Neuroscience, University of Sheffield , S10 2HQ Sheffield , UK .
  • Turner MR; o IRCCS Istituto Auxologico Italiano, Department of Neurology and Laboratory of Neuroscience, 20149 Milano, Italy .
  • Tüzün E; v Department "Dino Ferrari" Center, Department of Pathophysiology and Transplantation , Università degli Studi di Milano , 20122 Milano , Italy.
  • Yetimler B; a Department of Neurology , Ulm University Hospital , 89081 Ulm , Germany ;
  • Ludolph AC; j Nuffield Department of Clinical Neurosciences , University of Oxford , OX3 9DU Oxford , UK .
  • Otto M; u Neuroscience Department , Institute of Experimental Medical Research, Istanbul University , 34393 Istanbul , Turkey , and.
Amyotroph Lateral Scler Frontotemporal Degener ; 17(5-6): 404-13, 2016.
Article em En | MEDLINE | ID: mdl-27415180
ABSTRACT

OBJECTIVE:

Neurofilaments are leading neurochemical biomarkers for amyotrophic lateral sclerosis (ALS). Here, we investigated the effect of preanalytical factors on neurofilament concentrations in cerebrospinal fluid (CSF) in a "reverse" round-robin with 15 centers across Europe/U.S.

METHODS:

Samples from ALS and control patients (5/5 each center, n = 150) were analyzed for phosphorylated neurofilament heavy chain (pNfH) and neurofilament light chain (NfL) at two laboratories.

RESULTS:

CSF pNfH was increased (p < 0.05) in ALS in 10 out of 15 centers and NfL in 5 out of 12 centers. The coefficient of variation (CV%) of pNfH measurements between laboratories was 18.7 ± 19.1%. We calculated a diagnostic cut-off of >568.5 pg/mL for pNfH (sensitivity 78.7%, specificity 93.3%) and >1,431pg/mL for NfL (sensitivity 79.0%, specificity 86.4%).

CONCLUSION:

Values in ALS patients are already comparable between most centers, supporting eventual implementation into clinical routine. However, continuous quality control programs will be necessary for inclusion in the diagnostic work-up.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filamentos Intermediários / Biomarcadores / Esclerose Lateral Amiotrófica Tipo de estudo: Clinical_trials / Diagnostic_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged País/Região como assunto: America do norte / Europa Idioma: En Revista: Amyotroph Lateral Scler Frontotemporal Degener Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filamentos Intermediários / Biomarcadores / Esclerose Lateral Amiotrófica Tipo de estudo: Clinical_trials / Diagnostic_studies Limite: Adult / Aged / Female / Humans / Male / Middle aged País/Região como assunto: America do norte / Europa Idioma: En Revista: Amyotroph Lateral Scler Frontotemporal Degener Ano de publicação: 2016 Tipo de documento: Article