Isolation of a monoclonal antibody that recognizes the origin binding domain of JCV, but not SV40, large T-antigen.
Virology
; 497: 92-101, 2016 10.
Article
em En
| MEDLINE
| ID: mdl-27433780
ABSTRACT
Within immunocompromised populations, the JC polyomavirus is the cause of the often-fatal disease Progressive Multifocal Leukoencephalopathy (PML). JC virus encodes a protein, termed T-antigen (T-ag), which is essential for its replication and pathogenicity. Previous studies of JCV T-ag have, in general, used antibodies raised against SV40 T-ag. Unfortunately, SV40 T-ag is also detected in humans and therefore there have been concerns about cross-reactivity. To address this issue, we have isolated a monoclonal antibody that binds to the JCV, but not the SV40, T-ag origin-binding domain (OBD). Furthermore, the region on the surface of the JCV T-ag OBD that is recognized by the "anti-JCV OBD mAb" has been mapped. We also demonstrate that the "anti-JCV OBD mAb" will be a useful reagent for standard techniques (e.g., Westerns blots and ELISAs). Finally, we note that additional monoclonal Abs that are specific for the T-ags encoded by the other human polyomaviruses could be generated by adopting the approach described herein.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus JC
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Domínios e Motivos de Interação entre Proteínas
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Anticorpos Monoclonais
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Antígenos Virais de Tumores
Limite:
Animals
Idioma:
En
Revista:
Virology
Ano de publicação:
2016
Tipo de documento:
Article