Quantification of A. fumigatus-specific CD154+ T-cells-preanalytic considerations.
Med Mycol
; 55(2): 223-227, 2017 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-27486216
ABSTRACT
Fungal specific CD154+ T-cells have been described as a biomarker in invasive aspergillosis. The influence of sample storage on the detection of these cells was assessed. Six-hour delay prior to PBMC isolation is associated with an 18% decrease of cell viability and alterations of the cellular composition of the sample. This results in 87% reduction of CD154+ A. fumigatus specific cells due to reduced assay sensitivity and increased background values in unstimulated samples. If prompt cell measurement is not feasible, isolated PBMCs can be frozen (at -20°C and -80°C) and processed later with comparable assay reliability (mean value fresh vs. thawing 0.126, 0.133; Pearson-Coefficient 0.962).
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aspergillus fumigatus
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Manejo de Espécimes
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Subpopulações de Linfócitos T
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Ligante de CD40
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Aspergilose Pulmonar Invasiva
Limite:
Adult
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Female
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Humans
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Male
Idioma:
En
Revista:
Med Mycol
Ano de publicação:
2017
Tipo de documento:
Article