Effect of surface coating of KYb2F7:Tm(3+) on optical properties and biomedical applications.

ABSTRACT

This project aims to provide an insight on the effects of biocompatible polymers on the optical properties and the nanoparticle-cell interaction of KYb2F7Tm(3+) nanocrystals that exhibit strong near infrared (NIR) fluorescence. KYb2F7Tm(3+) nanocrystals were synthesized with a diameter of 20-30 nm and surface modified with poly(ethylene glycol), Pluronic(®) F-127, and poly(N-vinylpyrrolidone), due to the associated advantages. Some of these include biocompatibility and biodistribution in the instance of agglomeration and hydrophobicity as well as the addition of a targeting agent and drug loading by further functionalization. Despite the decrease in fluorescence intensity induced by the surface modification, thulium's emission fingerprint was easily detected. Moreover, surface modified KYb2F7Tm(3+) nanocrystals failed to induce a toxic response on endothelial cells following a 24 h uptake period up to concentrations of 100 µg ml(-1). In vitro toxicity and confocal imaging have demonstrated the versatility of these NIR fluorescence nanocrystals in biomedical imaging, drug delivery, and photodynamic therapy.