Validation of a novel real-time PCR assay for detection of HLA-B*15:02 allele for prevention of carbamazepine - Induced Stevens-Johnson syndrome/Toxic Epidermal Necrolysis in individuals of Asian ancestry.
Hum Immunol
; 77(12): 1140-1146, 2016 Dec.
Article
em En
| MEDLINE
| ID: mdl-27544047
ABSTRACT
OBJECTIVES:
Screening for the HLA-B*1502 allele has been recommended to prevent carbamazepine (CBZ) - induced Stevens-Johnson syndrome (SJS) and Toxic Epidermal Necrolysis (TEN) in individuals with Asian ancestry. We aimed, therefore, to develop and validate a robust and inexpensive method for detection of the HLA-B*1502 allele.METHODS:
Real-time PCR using TaqMan® probes followed by SYBR® Green was used to detect the HLA-B*1502 allele prior to treatment with CBZ therapy.RESULTS:
A total of 121 samples were tested. The assay has a sensitivity of 100% (95% CI 76.84-100.0%), a specificity of 100% (95% CI 96.61-100%), a positive predictive value of 100% (95% CI 76.84-100%) and a negative predictive value of 100.0% (95% CI 96.61-100.0%), respectively. There was 100% agreement between our results and genotyping using Luminex SSO/SBT/SSP. The lowest limit of detection of the TaqMan® probe is 0.05ng/µl and the SYBR® Green is 0.5ng/µl of DNA. The unit cost of using the TaqMan® probe followed by SYBR® Green is only $4.7 USD.CONCLUSION:
We developed a novel assay for the detection of the HLA-B*1502 allele, which is robust, inexpensive and suitable for screening individuals of Asian ancestry in the prevention of CBZ-induced SJS/TEN.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carbamazepina
/
Síndrome de Stevens-Johnson
/
Povo Asiático
/
Antígeno HLA-B15
/
Reação em Cadeia da Polimerase em Tempo Real
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Hum Immunol
Ano de publicação:
2016
Tipo de documento:
Article