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Persistence of fan-shaped keratocytes is a matrix-rigidity-dependent mechanism that requires α5ß1 integrin engagement.
Riaz, Maryam; Versaevel, Marie; Mohammed, Danahe; Glinel, Karine; Gabriele, Sylvain.
Afiliação
  • Riaz M; Mechanobiology &Soft Matter Group, Laboratoire Interfaces et Fluides Complexes, Centre d'Innovation et de Recherche en Matériaux Polymères (CIRMAP), Research Institute for Biosciences, Université de Mons, 20, Place du Parc, B-7000 Mons, Belgium.
  • Versaevel M; Mechanobiology &Soft Matter Group, Laboratoire Interfaces et Fluides Complexes, Centre d'Innovation et de Recherche en Matériaux Polymères (CIRMAP), Research Institute for Biosciences, Université de Mons, 20, Place du Parc, B-7000 Mons, Belgium.
  • Mohammed D; Mechanobiology &Soft Matter Group, Laboratoire Interfaces et Fluides Complexes, Centre d'Innovation et de Recherche en Matériaux Polymères (CIRMAP), Research Institute for Biosciences, Université de Mons, 20, Place du Parc, B-7000 Mons, Belgium.
  • Glinel K; Institute of Condensed Matter &Nanosciences, Bio &Soft Matter division, Universite Catholique de Louvain, Croix du Sud 1, box L7.04.02, B-1348 Louvain-la-Neuve, Belgium.
  • Gabriele S; Mechanobiology &Soft Matter Group, Laboratoire Interfaces et Fluides Complexes, Centre d'Innovation et de Recherche en Matériaux Polymères (CIRMAP), Research Institute for Biosciences, Université de Mons, 20, Place du Parc, B-7000 Mons, Belgium.
Sci Rep ; 6: 34141, 2016 Sep 28.
Article em En | MEDLINE | ID: mdl-27678055
Despite the importance of matrix rigidity on cell functions, many aspects of the mechanosensing process in highly migratory cells remain elusive. Here, we studied the migration of highly motile keratocytes on culture substrates with similar biochemical properties and rigidities spanning the range between soft tissues (~kPa) and stiff culture substrates (~GPa). We show that morphology, polarization and persistence of motile keratocytes are regulated by the matrix stiffness over seven orders of magnitude, without changing the cell spreading area. Increasing the matrix rigidity leads to more F-actin in the lamellipodia and to the formation of mature contractile actomyosin fibers that control the cell rear retraction. Keratocytes remain rounded and form nascent adhesions on compliant substrates, whereas large and uniformly distributed focal adhesions are formed on fan-shaped keratocytes migrating on rigid surfaces. By combining poly-L-lysine, fibronectin and vitronectin coatings with selective blocking of αvß3 or α5ß1 integrins, we show that αVß3 integrins permit the spreading of keratocytes but are not sufficient for polarization and rigidity sensing that require the engagement of α5ß1 integrins. Our study demonstrates a matrix rigidity-dependent regulation of the directional persistence in motile keratocytes and refines the role of αvß3 and α5ß1 integrins in the molecular clutch model.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Sci Rep Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Sci Rep Ano de publicação: 2016 Tipo de documento: Article