Studying Wnt Signaling During Patterning of Conducting Airways.
J Vis Exp
; (116)2016 10 16.
Article
em En
| MEDLINE
| ID: mdl-27805581
ABSTRACT
Wnt signaling pathways play critical roles during development of the respiratory tract. Defining precise mechanisms of differentiation and morphogenesis controlled by Wnt signaling is required to understand how tissues are patterned during normal development. This knowledge is also critical to determine the etiology of birth defects such as lung hypoplasia and tracheobronchomalacia. Analysis of earliest stages of development of respiratory tract imposes challenges, as the limited amount of tissue prevents the performance of standard protocols better suited for postnatal studies. In this paper, we discuss methodologies to study cell differentiation and proliferation in the respiratory tract. We describe techniques such as whole mount staining, processing of the tissue for confocal microscopy and immunofluorescence in paraffin sections applied to developing tracheal lung. We also discuss methodologies for the study of tracheal mesenchyme differentiation, in particular cartilage formation. Approaches and techniques discussed in the current paper circumvent the limitation of material while working with embryonic tissue, allowing for a better understanding of the patterning process of developing conducting airways.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sistema Respiratório
/
Coloração e Rotulagem
/
Inclusão do Tecido
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Padronização Corporal
/
Via de Sinalização Wnt
Limite:
Animals
Idioma:
En
Revista:
J Vis Exp
Ano de publicação:
2016
Tipo de documento:
Article