Patterning of Endothelial Cells and Mesenchymal Stem Cells by Laser-Assisted Bioprinting to Study Cell Migration.
Biomed Res Int
; 2016: 3569843, 2016.
Article
em En
| MEDLINE
| ID: mdl-27833916
ABSTRACT
Tissue engineering of large organs is currently limited by the lack of potent vascularization in vitro. Tissue-engineered bone grafts can be prevascularized in vitro using endothelial cells (ECs). The microvascular network architecture could be controlled by printing ECs following a specific pattern. Using laser-assisted bioprinting, we investigated the effect of distance between printed cell islets and the influence of coprinted mesenchymal cells on migration. When printed alone, ECs spread out evenly on the collagen hydrogel, regardless of the distance between cell islets. However, when printed in coculture with mesenchymal cells by laser-assisted bioprinting, they remained in the printed area. Therefore, the presence of mesenchymal cell is mandatory in order to create a pattern that will be conserved over time. This work describes an interesting approach to study cell migration that could be reproduced to study the effect of trophic factors.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Movimento Celular
/
Células-Tronco Mesenquimais
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Células Endoteliais da Veia Umbilical Humana
/
Bioimpressão
/
Lasers
Limite:
Female
/
Humans
/
Male
Idioma:
En
Revista:
Biomed Res Int
Ano de publicação:
2016
Tipo de documento:
Article