UNR/CSDE1 Drives a Post-transcriptional Program to Promote Melanoma Invasion and Metastasis.
Cancer Cell
; 30(5): 694-707, 2016 Nov 14.
Article
em En
| MEDLINE
| ID: mdl-27908735
RNA binding proteins (RBPs) modulate cancer progression through poorly understood mechanisms. Here we show that the RBP UNR/CSDE1 is overexpressed in melanoma tumors and promotes invasion and metastasis. iCLIP sequencing, RNA sequencing, and ribosome profiling combined with in silico studies unveiled sets of pro-metastatic factors coordinately regulated by UNR as part of RNA regulons. In addition to RNA steady-state levels, UNR was found to control many of its targets at the level of translation elongation/termination. Key pro-oncogenic targets of UNR included VIM and RAC1, as validated by loss- and gain-of-function studies. Our results identify UNR as an oncogenic modulator of melanoma progression, unravel the underlying molecular mechanisms, and identify potential targets for this therapeutically challenging malignancy.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vimentina
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Regulação para Cima
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Proteínas de Ligação a RNA
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Proteínas rac1 de Ligação ao GTP
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Proteínas de Ligação a DNA
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Melanoma
Tipo de estudo:
Prognostic_studies
Limite:
Animals
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Humans
Idioma:
En
Revista:
Cancer Cell
Ano de publicação:
2016
Tipo de documento:
Article