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Serum and knee synovial fluid matrixmetalloproteinase-13 and tumor necrosis factor-alpha levels in patients with late stage osteoarthritis.
Özler, Kenan; Aktas, Erdem; Atay, Çigdem; Yilmaz, Baris; Arikan, Murat; Güngör, Safak.
Afiliação
  • Özler K; Dr. Abdurrahman Yurtaslan Oncology Training and Research Hospital, Department of Orthopedics, Ankara, Turkey.
  • Aktas E; Dr. Abdurrahman Yurtaslan Onkoloji Training and Research Hospital, Ankara, Turkey. Electronic address: drerdem2007@gmail.com.
  • Atay Ç; Dr. Abdurrahman Yurtaslan Oncology Training and Research Hospital, Department of Biochemistry, Ankara, Turkey.
  • Yilmaz B; Fatih Sultan Mehmet Training and Research Hospital, Department of Orthopedics, Istanbul, Turkey.
  • Arikan M; Dr. Abdurrahman Yurtaslan Oncology Training and Research Hospital, Department of Orthopedics, Ankara, Turkey.
  • Güngör S; Dr. Abdurrahman Yurtaslan Oncology Training and Research Hospital, Department of Orthopedics, Ankara, Turkey.
Acta Orthop Traumatol Turc ; 50(6): 670-673, 2016 Dec.
Article em En | MEDLINE | ID: mdl-27932045
OBJECTIVE: To compare the levels of MMP-13 and TNF-α in late stage osteoarthritis, define their predominant pathways and investigate their correlation with McMaster Universities Arthritis Index scores. PATIENTS AND METHODS: A total of 42 patients (mean age 64 ± 8.8) with grade 3 and grade 4 knee osteoarthritis according to Kellegren- Lawrence criteria and who were scheduled for total knee arthroplasty were enrolled in the study. TNF-alpha and MMP-13 levels were measured preoperatively from venous blood samples and intraoperatively from knee synovial fluid via ELISA. Preoperative and 1 month postoperative knee functions were assessed by McMaster Universities Arthritis Index. RESULTS: Grade 4 synovial fluid MMP-13 (4.76 ± 5.82) was elevated compared to grade 3 (3.95 ± 4.45) (p = 0.438), whereas grade 3 serum MMP-13 (1.128 ± 0.308) was found elevated compared to grade 4 (1.038 ± 0.204) (p = 0.430). Grade 4 serum TNF-α (0.253 ± 0.277) was elevated compared to grade 3 (0.206 ± 0.219) whereas grade 3 synovial fluid TNF-α (0.129 ± 0.052) was elevated compared to grade 4 (0.118 ± 0.014). Positive correlation was observed between synovial fluid MMP-13 levels and postoperative WOMAC scores. Mean serum TNF-α level (0.226 ± 0.246 pg/ml) was found higher compared to synovial level (0.124 ± 1.59), synovial MMP-13 level (4.31 ± 1.24) was found higher compared to serum level (1.089 ± 1.519). CONCLUSION: Despite the systemic increase in TNF-α levels concordant with osteoarthritis grade, MMP-13 levels are elevated via local manner with a significant correlation with WOMAC scores. LEVEL OF EVIDENCE: Level IV, Diagnostic study.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Líquido Sinovial / Fator de Necrose Tumoral alfa / Artroplastia do Joelho / Osteoartrite do Joelho / Metaloproteinase 13 da Matriz Tipo de estudo: Diagnostic_studies Limite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Acta Orthop Traumatol Turc Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Líquido Sinovial / Fator de Necrose Tumoral alfa / Artroplastia do Joelho / Osteoartrite do Joelho / Metaloproteinase 13 da Matriz Tipo de estudo: Diagnostic_studies Limite: Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Acta Orthop Traumatol Turc Ano de publicação: 2016 Tipo de documento: Article