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Generation of human iPSCs from urine derived cells of a patient with a novel homozygous PAI-1 mutation.
Afzal, Muhammad Zeeshan; Gartz, Melanie; Klyachko, Ekaterina A; Khan, Sadiya Sana; Shah, Sanjiv J; Gupta, Sweta; Shapiro, Amy D; Vaughan, Douglas E; Strande, Jennifer L.
Afiliação
  • Afzal MZ; Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Gartz M; Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Klyachko EA; Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.
  • Khan SS; Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.
  • Shah SJ; Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.
  • Gupta S; Indiana Hemophilia and Thrombosis Center, Indianapolis, IN, United States.
  • Shapiro AD; Indiana Hemophilia and Thrombosis Center, Indianapolis, IN, United States.
  • Vaughan DE; Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.
  • Strande JL; Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States. Electronic address: jstrande@mcw.edu.
Stem Cell Res ; 17(3): 657-660, 2016 11.
Article em En | MEDLINE | ID: mdl-27934602
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from subject with a novel homozygous Plasminogen Activator Inhibitor-1 (PAI-1 null) mutation. The Sendai virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the PAI-1 UCs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Inibidor 1 de Ativador de Plasminogênio / Células-Tronco Pluripotentes Induzidas Limite: Female / Humans Idioma: En Revista: Stem Cell Res Ano de publicação: 2016 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Inibidor 1 de Ativador de Plasminogênio / Células-Tronco Pluripotentes Induzidas Limite: Female / Humans Idioma: En Revista: Stem Cell Res Ano de publicação: 2016 Tipo de documento: Article