A Novel Tool to Measure Extracellular Glutamate in the Zebrafish Nervous System In Vivo.
Zebrafish
; 14(3): 284-286, 2017 06.
Article
em En
| MEDLINE
| ID: mdl-28027028
ABSTRACT
Glutamate is the major excitatory neurotransmitter in the brain. Its release and eventual recycling are key to rapid sustained neural activity. We have paired the gfap promoter region with the glutamate reporter molecule, iGluSnFR, to drive expression in glial cells throughout the nervous system. Tg(gfapiGluSnFR) is expressed on the glial membrane of Müller glia cells in the retina, which rapidly respond to stimulation and the release of extracellular glutamate. As glial cells are associated with most, if not all, synapses, Tg(gfapiGluSnFR) is a novel and exciting tool to measure neuronal activity and extracellular glutamate dynamics in many regions of the nervous system.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes de Fusão
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Peixe-Zebra
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Ácido Glutâmico
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Proteínas de Escherichia coli
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Proteínas de Fluorescência Verde
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Sistema Nervoso
Limite:
Animals
Idioma:
En
Revista:
Zebrafish
Ano de publicação:
2017
Tipo de documento:
Article