ROS accumulation and antiviral defence control by microRNA528 in rice.
Nat Plants
; 3: 16203, 2017 01 06.
Article
em En
| MEDLINE
| ID: mdl-28059073
ABSTRACT
MicroRNAs (miRNAs) are key regulators of plant-pathogen interactions. Modulating miRNA function has emerged as a new strategy to produce virus resistance traits1-5. However, the miRNAs involved in antiviral defence and the underlying mechanisms remain largely elusive. We previously demonstrated that sequestration by Argonaute (AGO) proteins plays an important role in regulating miRNA function in antiviral defence pathways6. Here we reveal that cleavage-defective AGO18 complexes sequester microRNA528 (miR528) upon viral infection. We show that miR528 negatively regulates viral resistance in rice by cleaving L-ascorbate oxidase (AO) messenger RNA, thereby reducing AO-mediated accumulation of reactive oxygen species. Upon viral infection, miR528 becomes preferentially associated with AGO18, leading to elevated AO activity, higher basal reactive oxygen species accumulation and enhanced antiviral defence. Our findings reveal a mechanism in which antiviral defence is boosted through suppression of an miRNA that negatively regulates viral resistance. This mechanism could be manipulated to engineer virus-resistant crop plants.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
/
Oryza
/
Espécies Reativas de Oxigênio
/
Tenuivirus
/
MicroRNAs
/
Resistência à Doença
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Nat Plants
Ano de publicação:
2017
Tipo de documento:
Article