Characterization of the interface of binary mixed DOPC:DOPS liposomes in water: The impact of charge condensation.
J Chem Phys
; 146(4): 044701, 2017 Jan 28.
Article
em En
| MEDLINE
| ID: mdl-28147550
ABSTRACT
Solutions of liposomes composed of binary mixtures of anionic dioleoylphosphatidylserine (DOPS) and zwitterionic dioleoylphosphatidylcholine (DOPC) are investigated with label-free angle-resolved (AR) second harmonic scattering (SHS) and electrophoretic mobility measurements. The membrane surface potential is extracted from the AR-SHS response. The surface potential changes from -10 to -145 mV with varying DOPS content ( from 0% to 100%) and levels off already at ⼠10 % DOPS content. The ζ-potential shows the same trend but with a drastically lower saturation value (-44 mV). This difference is explained by the formation of a condensed layer of Na+ counterions around the outer leaflet of the liposome as predicted by charge condensation theories for polyelectrolyte systems.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fosfatidilcolinas
/
Fosfatidilserinas
/
Lipossomos
Idioma:
En
Revista:
J Chem Phys
Ano de publicação:
2017
Tipo de documento:
Article