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Superresolution Imaging Identifies That Conventional Trafficking Pathways Are Not Essential for Endoplasmic Reticulum to Outer Mitochondrial Membrane Protein Transport.
Salka, Kyle; Bhuvanendran, Shivaprasad; Wilson, Kassandra; Bozidis, Petros; Mehta, Mansi; Rainey, Kristin; Sesaki, Hiromi; Patterson, George H; Jaiswal, Jyoti K; Colberg-Poley, Anamaris M.
Afiliação
  • Salka K; Center for Genetic Medicine Research, Children's National Health System, 111 Michigan Ave, NW, Washington, DC, 20010, USA.
  • Bhuvanendran S; Center for Genetic Medicine Research, Children's National Health System, 111 Michigan Ave, NW, Washington, DC, 20010, USA.
  • Wilson K; Center for Genetic Medicine Research, Children's National Health System, 111 Michigan Ave, NW, Washington, DC, 20010, USA.
  • Bozidis P; Center for Genetic Medicine Research, Children's National Health System, 111 Michigan Ave, NW, Washington, DC, 20010, USA.
  • Mehta M; Laboratory of Microbiology, Department of Medicine, School of Health Sciences, University of Ioannina, Ioannina, 45500, Greece.
  • Rainey K; Center for Genetic Medicine Research, Children's National Health System, 111 Michigan Ave, NW, Washington, DC, 20010, USA.
  • Sesaki H; Section on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, 20892, USA.
  • Patterson GH; Department of Cell Biology, Johns Hopkins University School of Medicine Hunterian 111, 725 N. Wolfe Street, Baltimore, MD, 21205, USA.
  • Jaiswal JK; Section on Biophotonics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, 20892, USA.
  • Colberg-Poley AM; Center for Genetic Medicine Research, Children's National Health System, 111 Michigan Ave, NW, Washington, DC, 20010, USA. jkjaiswal@childrensnational.org.
Sci Rep ; 7(1): 16, 2017 02 02.
Article em En | MEDLINE | ID: mdl-28154412
ABSTRACT
Most nuclear-encoded mitochondrial proteins traffic from the cytosol to mitochondria. Some of these proteins localize at mitochondria-associated membranes (MAM), where mitochondria are closely apposed with the endoplasmic reticulum (ER). We have previously shown that the human cytomegalovirus signal-anchored protein known as viral mitochondria-localized inhibitor of apoptosis (vMIA) traffics from the ER to mitochondria and clusters at the outer mitochondrial membrane (OMM). Here, we have examined the host pathways by which vMIA traffics from the ER to mitochondria and clusters at the OMM. By disruption of phosphofurin acidic cluster sorting protein 2 (PACS-2), mitofusins (Mfn1/2), and dynamin related protein 1 (Drp1), we find these conventional pathways for ER to the mitochondria trafficking are dispensable for vMIA trafficking to OMM. Instead, mutations in vMIA that change its hydrophobicity alter its trafficking to mitochondria. Superresolution imaging showed that PACS-2- and Mfn-mediated membrane apposition or hydrophobic interactions alter vMIA's ability to organize in nanoscale clusters at the OMM. This shows that signal-anchored MAM proteins can make use of hydrophobic interactions independently of conventional ER-mitochondria pathways to traffic from the ER to mitochondria. Further, vMIA hydrophobic interactions and ER-mitochondria contacts facilitate proper organization of vMIA on the OMM.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Imediatamente Precoces / Retículo Endoplasmático / Membranas Mitocondriais / Proteínas de Membrana Limite: Animals / Humans Idioma: En Revista: Sci Rep Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Imediatamente Precoces / Retículo Endoplasmático / Membranas Mitocondriais / Proteínas de Membrana Limite: Animals / Humans Idioma: En Revista: Sci Rep Ano de publicação: 2017 Tipo de documento: Article