Detection of 3,4-dihydroxyphenylglycolaldehyde in human brain by high-performance liquid chromatography.
Anal Biochem
; 180(1): 79-84, 1989 Jul.
Article
em En
| MEDLINE
| ID: mdl-2817346
ABSTRACT
The monoamine oxidase A metabolite of noradrenaline, 3,4-dihydroxyphenylglycolaldehyde, is the precursor of 3,4-dihydroxymandelic acid, and 3,4-dihydroxyphenylglycol, metabolites of noradrenaline. Owing to difficulties in purifying this aldehyde, it has not been previously characterized or identified in biological sources. This paper describes an enzymatic synthesis, purification, and characterization of 3,4-dihydroxyphenylglycolaldehyde. The aldehyde metabolite is identified in postmortem human brain using high-performance liquid chromatography and electrochemical detection. We estimate the concentration in human hippocampus to be 0.164 +/- 0.05 nmol/g. The importance of this aldehyde metabolite of noradrenaline is discussed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Química Encefálica
Tipo de estudo:
Diagnostic_studies
Limite:
Aged
/
Aged80
/
Humans
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
1989
Tipo de documento:
Article