Use of dual priming oligonucleotide system-based multiplex RT-PCR combined with high performance liquid chromatography assay for simultaneous detection of five enteric viruses associated with acute enteritis.
J Virol Methods
; 243: 80-82, 2017 05.
Article
em En
| MEDLINE
| ID: mdl-28185831
ABSTRACT
In this study, a specific and sensitive method for simultaneous detection of human astrovirus, human rotavirus, norovirus, sapovirus and enteric adenovirus associated with acute enteritis was developed, based on the specific dual priming oligonucleotide (DPO) system and the sensitive high-performance liquid chromatography (HPLC) analysis. The DPO system-based multiplex reverse transcription-polymerase chain reaction (RT-PCR) combined with HPLC assay was more sensitive than agarose gel electrophoresis analysis and real-time SYBR Green PCR assay, and showed a specificity of 100% and sensitivity of 96%-100%. The high sensitivity and specificity of the assay indicates its great potential to be a useful tool for the accurate diagnosis of enteric virus infections.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Viroses
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Cromatografia Líquida de Alta Pressão
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Reação em Cadeia da Polimerase Via Transcriptase Reversa
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Técnicas de Diagnóstico Molecular
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Enterite
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Reação em Cadeia da Polimerase Multiplex
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
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Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
2017
Tipo de documento:
Article