Astragaloside IV inhibits isoprenalineinduced cardiac fibrosis by targeting the reactive oxygen species/mitogenactivated protein kinase signaling axis.
Mol Med Rep
; 15(4): 1765-1770, 2017 Apr.
Article
em En
| MEDLINE
| ID: mdl-28260010
ABSTRACT
Cardiac fibrosis is considered an important pathological mechanism in the progression of cardiac remodeling and heart failure. Astragaloside IV (AsIV) is a major active ingredient in Astragalus membranaceus. In a preliminary experiment, it was demonstrated that this naturally occurring substance exhibited cardioprotective effects via preventing cardiomyocyte hypertrophy and apoptosis. The present study aimed to investigate the effects of AsIV on ßadrenergic receptor (ßAR)mediated cardiac fibrosis, and the associated mechanism. Cell Counting Kit8 (CCK8) assay was used to examine the proliferation of rat cardiac fibroblast (CF) cultures. Collagen I secretion was detected by ELISA. Dihydroethidium was used to determine intracellular ROS levels. Western blotting was used to examine the expression level of total and phosphorylated mitogenactivated protein kinases (MAPKs). In the present study, the effects of AsIV on ßadrenergic receptor (ßAR) mediated cardiac fibrosis were investigated, and the associated mechanism was revealed. Isoprenaline (ISO) is a selective ßAR agonist, and treatment with AsIV significantly inhibited (ISO)triggered cardiac fibroblast proliferation and type I collagen synthesis. In addition, ISO resulted in a significant elevation of reactive oxygen species (ROS) levels and phosphorylation of the three profibrotic MAPKs, namely extracellular signalregulated kinase, p38MAPK and cJun Nterminal kinase. AsIV effectively reversed the aforementioned ISOinduced alterations. In addition, Nacetylcysteine, a typical ROS scavenger, mimicked the inhibitory effects of AsIV on MAPK activation. The present study demonstrated that AsIV may inhibit ISOinduced cardiac fibrosis by suppressing ROSmediated MAPK activation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saponinas
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Triterpenos
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Sistema de Sinalização das MAP Quinases
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Fibroblastos
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Coração
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Miocárdio
Limite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Ano de publicação:
2017
Tipo de documento:
Article