Relocation of NPM Affects the Malignant Phenotypes of Hepatoma SMMC-7721 Cells.
J Cell Biochem
; 118(10): 3225-3236, 2017 10.
Article
em En
| MEDLINE
| ID: mdl-28262969
ABSTRACT
Nucleophosmin(NPM), heavily implicated in diverse solid tumors, is an important multifunctional protein mainly located in the nucleolus. Our previous study confirmed that NPM can also localize and accumulate in the cytoplasm of liver cancer cells. However, the role of cytoplasmic NPM (NPMc +) is unclear. Here, we showed that both nucleolar NPM and NPMc+ could promote cell proliferation, although the effect of NPMc+ was weaker than that of NPM. Cell adhesion ability of hepatoma cells was significantly reduced to a greater extent by NPMc+ expression. Nucleolar NPM enhanced cell migration and invasion, whereas NPMc+ impeded cell migration and invasion. The investigation of NPM interactional proteins by proteomic method demonstrated that the NPM was involved in multiple biological processes. By contrast, the interactional proteins of NPMc+ were mainly implicated in tRNA amino acylation regulation. The interactional network of NPMc+ was significantly small and simple. These results suggested that relocation of NPM altered its interactional network and consequently disturbed the primary functions, including cell proliferation, adhesion, migration, and invasion. NPM plays a promotional role in cancer and the reducing relocation may be a potential therapeutic target for hepatocellular carcinoma. J. Cell. Biochem. 118 3225-3236, 2017. © 2017 Wiley Periodicals, Inc.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Nucleares
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Movimento Celular
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Carcinoma Hepatocelular
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Proliferação de Células
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Neoplasias Hepáticas
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Proteínas de Neoplasias
Limite:
Humans
Idioma:
En
Revista:
J Cell Biochem
Ano de publicação:
2017
Tipo de documento:
Article