Solubilization and characterization of calcitonin gene-related peptide binding site from porcine spinal cord.
J Neurochem
; 50(2): 480-5, 1988 Feb.
Article
em En
| MEDLINE
| ID: mdl-2826696
ABSTRACT
The binding site for calcitonin gene-related peptide (CGRP) was solubilized with 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (CHAPS) in an active form from porcine spinal cord. 125I-labeled human alpha-CGRP (125I-CGRP) binding to the solubilized protein was determined by filtration using a GF/B glass filter. The maximal binding activity (approximately 60% of the crude membrane fraction) was obtained with 5 mM CHAPS. 125I-CGRP binding to the solubilized protein was of high affinity, saturability, and high specificity, having KD and Bmax values of 3.69 pM and 338 fmol/mg of protein, respectively. The binding activity was eluted in a single peak with a molecular mass of 400,000 daltons by gel filtration on TSK gel G4000SW. These results suggest that the solubilized protein may be responsible for the specific binding site.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Medula Espinal
/
Neuropeptídeos
/
Receptores de Superfície Celular
Limite:
Animals
Idioma:
En
Revista:
J Neurochem
Ano de publicação:
1988
Tipo de documento:
Article