Dipeptidyl peptidase IV is involved in the cellulose-responsive induction of cellulose biomass-degrading enzyme genes in Aspergillus aculeatus.
Biosci Biotechnol Biochem
; 81(6): 1227-1234, 2017 Jun.
Article
em En
| MEDLINE
| ID: mdl-28290772
ABSTRACT
We screened for factors involved in the cellulose-responsive induction of cellulose biomass-degrading enzyme genes from approximately 12,000 Aspergillus aculeatus T-DNA insertion mutants harboring a transcriptional fusion between the FIII-avicelase gene (cbhI) promoter and the orotidine 5'-monophosphate decarboxylase gene. Analysis of 5-fluoroorodic acid (5-FOA) sensitivity, cellulose utilization, and cbhI expression of the mutants revealed that a mutant harboring T-DNA at the dipeptidyl peptidase IV (dppIV) locus had acquired 5-FOA resistance and was deficient in cellulose utilization and cbhI expression. The deletion of dppIV resulted in a significant reduction in the cellulose-responsive expression of both cbhI as well as genes controlled by XlnR-independent and XlnR-dependent signaling pathways at an early phase in A. aculeatus. In contrast, the dppIV deletion did not affect the xylose-responsive expression of genes under the control of XlnR. These results demonstrate that DppIV participates in cellulose-responsive induction in A. aculeatus.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Orotidina-5'-Fosfato Descarboxilase
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Aspergillus
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Proteínas Fúngicas
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Regulação Fúngica da Expressão Gênica
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Celulose
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Dipeptidil Peptidase 4
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Celulases
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Ano de publicação:
2017
Tipo de documento:
Article