Microarray-based analysis of gene expression profiles in peripheral blood of patients with acute primary angle closure.
Ophthalmic Genet
; 38(6): 520-526, 2017 12.
Article
em En
| MEDLINE
| ID: mdl-28323501
ABSTRACT
BACKGROUND:
We investigated the expression of molecules in peripheral blood mononuclear cells (PBMCs) and plasma of patients with acute primary angle closure (APAC). MATERIALS ANDMETHODS:
Peripheral blood was collected from patients with APAC (n = 10) and age-matched controls (n = 5). The gene transcription profile was analyzed in PBMCs using microarrays and validated by real-time reverse transcription polymerase chain reaction (RT-PCR). The levels of secreted proteins were evaluated in plasma by ELISA.RESULTS:
347 gene transcripts were up-regulated by 2-fold or more, and 696 transcripts down-regulated 2-fold or more in PBMCs from patients compared to controls. The most highly up-regulated gene was thrombospondin-1 (TSP-1, 8.66-fold increase), and the most down-regulated gene was prostaglandin-endoperoxide synthase 2 (PTGS2, 9.09-fold decrease). Real-time RT-PCR assay confirmed the increase of TSP-1 and the decrease of PTGS2 in PBMCs of patients. ELISA revealed that the levels of TSP-1 and active transforming growth factor (TGF)-ß1 that is activated by TSP-1 were elevated in plasma of patients, while the level of prostaglandin E2 (PGE2) that is converted by PTGS2 was reduced. The plasma level of TSP-1 was positively correlated with that of active TGF-ß1.CONCLUSIONS:
Our data suggest that the molecular network including TSP-1, TGF-ß1, and PGE2 might be involved in the pathogenesis of APAC and PACG.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dinoprostona
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Glaucoma de Ângulo Fechado
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Regulação da Expressão Gênica
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Trombospondina 1
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Fator de Crescimento Transformador beta1
Limite:
Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Ophthalmic Genet
Ano de publicação:
2017
Tipo de documento:
Article