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Application of iTRAQ Shotgun Proteomics for Measurement of Brain Proteins in Studies of Psychiatric Disorders.
Núñez, Erika Velásquez; Guest, Paul C; Martins-de-Souza, Daniel; Domont, Gilberto Barbosa; Nogueira, Fábio César Sousa.
Afiliação
  • Núñez EV; Laboratory of Protein Chemistry - Proteomics Unit, Chemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Guest PC; Department of Biochemistry and Tissue Biology, Laboratory of Neuroproteomics, Institute of Biology, University of Campinas, CidadeUniversitáriaZeferinoVaz, Campinas, Brazil.
  • Martins-de-Souza D; Department of Biochemistry and Tissue Biology, Laboratory of Neuroproteomics, Institute of Biology, University of Campinas, CidadeUniversitáriaZeferinoVaz, Campinas, Brazil.
  • Domont GB; Laboratory of Protein Chemistry - Proteomics Unit, Chemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
  • Nogueira FC; Laboratory of Protein Chemistry - Proteomics Unit, Chemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil. fabiocsn@ufrj.br.
Adv Exp Med Biol ; 974: 219-227, 2017.
Article em En | MEDLINE | ID: mdl-28353239
Shotgun proteomics has been used successfully for more than two decades for measurement of proteins from diverse biological systems. This has led to new insights and identification of potential biomarkers and drug targets for numerous medical conditions. The advent of mass-labeling approaches such as isobaric tagging for relative and absolute quantitation (iTRAQ) has allowed multiplexing of samples to improve the accuracy and throughput of experiments and the reduction of costs. Here, we describe a detailed iTRAQ mass spectrometry protocol for simultaneous analysis of four proteomes from postmortem brain tissue extracts. In addition, a post-labeling procedure for peptide fractionation is presented.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Química Encefálica / Espectrometria de Massas em Tandem / Transtornos Mentais / Proteínas do Tecido Nervoso Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Adv Exp Med Biol Ano de publicação: 2017 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Química Encefálica / Espectrometria de Massas em Tandem / Transtornos Mentais / Proteínas do Tecido Nervoso Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: Adv Exp Med Biol Ano de publicação: 2017 Tipo de documento: Article